Hotspot

trpG-ccs1

Genome · 7830-8172 · 343 bp

Back to Gracilaria

Pi 0.315

MarkerSeek score 73.10

Primer available yes

Label trpG-ccs1

Feature Metadata

label_name: trpG-ccs1
feature_type: igs
region: Genome
length_bp: 343
variable_sites: 252
pi: 0.315
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trpG-ccs1_p1	TGGGAGTTATATCTGGGCGT	TCTCAAATCTGGGTCAACAGT	50.0	42.9	57.9	56.9	1170-1174 bp	0.117647	31.3
trpG-ccs1_p2	TGGGAGTTATATCTGGGCGT	TGGTCATCCCAGATTCCTATCA	50.0	45.5	57.9	58.1	1440 bp	0.058824	25.1
trpG-ccs1_p3	CAGCGCGTTATCACAGTTTG	TGGTCATCCCAGATTCCTATCA	50.0	45.5	58.1	58.1	939 bp	0.058824	24.8
trpG-ccs1_p4	TGGGAGTTATATCTGGGCGT	GCAGGTTTCACTAATAGAGCCA	50.0	45.5	57.9	58.1	1128 bp	0.058824	24.6
trpG-ccs1_p5	TGGGAGTTATATCTGGGCGT	AGCAGGTTTCACTAATAGAGCC	50.0	45.5	57.9	57.9	1129 bp	0.058824	24.4