Hotspot

rrn5-rps6

Genome · 4946-5274 · 329 bp

Back to Gracilaria

Pi 0.35

MarkerSeek score 72.30

Primer available yes

Label rrn5-rps6

Feature Metadata

label_name: rrn5-rps6
feature_type: igs
region: Genome
length_bp: 329
variable_sites: 268
pi: 0.35
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rps6_p1	TTATAGCGCAGTGGAACCAC	CCCTATACCAGACCGTCCAT	50.0	55.0	58.0	58.0	1009-1079 bp	0.411765	68.2
rrn5-rps6_p2	ATCTCCCCCAAGAGTTCACA	CCCTATACCAGACCGTCCAT	50.0	55.0	58.0	58.0	1500-1570 bp	0.411765	68.0
rrn5-rps6_p3	TCTCCCCCAAGAGTTCACAT	CCCTATACCAGACCGTCCAT	50.0	55.0	58.0	58.0	1499-1569 bp	0.411765	68.0
rrn5-rps6_p4	CAAGTCGAGCAGAGACGAAA	CCCTATACCAGACCGTCCAT	50.0	55.0	57.9	58.0	1600-1670 bp	0.411765	68.0
rrn5-rps6_p5	AGGGAAGCTTTTTGGGAAGG	CCCTATACCAGACCGTCCAT	50.0	55.0	58.0	58.0	936-1006 bp	0.235294	61.2