Hotspot

psbA

LSC · 41871-42932 · 1062 bp

Back to Goudaea

Pi 0.00188

MarkerSeek score 48.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTCATATACATTAAGACTTCTGTC	GTGCTTGGGAGTCCTTGAAA	29.2	50.0	52.2	58.0	1129 bp	1.0	40.1
psbA_p2	TTCATATACATTAAGACTTCTGTC	AATACGTGTGCTTGGGAGTC	29.2	50.0	52.2	57.9	1136 bp	1.0	40.1
psbA_p3	ATTCATATACATTAAGACTTCTGTC	GTGCTTGGGAGTCCTTGAAA	28.0	50.0	52.8	58.0	1130 bp	1.0	40.1
psbA_p4	ATTCATATACATTAAGACTTCTGTC	AATACGTGTGCTTGGGAGTC	28.0	50.0	52.8	57.9	1137 bp	1.0	40.1
psbA_p5	CAAGATATTGGGTATTATTCCTTAA	GTGCTTGGGAGTCCTTGAAA	28.0	50.0	52.7	58.0	1154 bp	1.0	40.1