Hotspot

atpF

LSC · 53183-54578 · 1396 bp

Back to Goudaea

Pi 0.00788

MarkerSeek score 54.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1396
variable_sites: 11
pi: 0.00788
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTAATAGAA	45.0	50.0	57.4	57.5	1570 bp	1.0	71.4
atpF_p2	GGCTCGAAGGGTTACCATTA	GGGCGAGCGAAGTAATAGAA	50.0	50.0	57.0	57.5	1558 bp	1.0	68.6
atpF_p3	ACTAATTTCGTCGGCTCGAA	AAGGGGCGAGCGAAGTAATA	45.0	50.0	57.4	58.9	1573 bp	1.0	68.4
atpF_p4	GGCTCGAAGGGTTACCATTAG	GGGCGAGCGAAGTAATAGAA	52.4	50.0	58.2	57.5	1558 bp	1.0	67.2
atpF_p5	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTAATAGAAA	45.0	47.6	57.4	58.2	1570 bp	1.0	66.0