Hotspot

ycf15-trnL-CAA

IRa · 137727-138475 · 749 bp

Back to Goudaea

Pi 0
MarkerSeek score 61.60
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRa
length_bp
749
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 GGCGAACAACCAGAGTACTC AAATGGTAGACACGCGAGAC 55.0 50.0 58.3 58.0 913-935 bp 1.0 79.5
ycf15-trnL-CAA_p2 GGCGAACAACCAGAGTACTC TGAAATGGTAGACACGCGAG 55.0 50.0 58.3 58.0 915-937 bp 1.0 79.5
ycf15-trnL-CAA_p3 GCGAACAACCAGAGTACTCA AAATGGTAGACACGCGAGAC 50.0 50.0 57.6 58.0 912-934 bp 1.0 78.4
ycf15-trnL-CAA_p4 GCGAACAACCAGAGTACTCA TGAAATGGTAGACACGCGAG 50.0 50.0 57.6 58.0 914-936 bp 1.0 78.4
ycf15-trnL-CAA_p5 GGCGAACAACCAGAGTACTC TGGTGAAATGGTAGACACGC 55.0 50.0 58.3 58.2 918-940 bp 1.0 78.0