Hotspot

rpl22-rps19

IRa · 127824-127942 · 119 bp

Back to Goudaea

Pi 0.0168

MarkerSeek score 62.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRa
length_bp: 119
variable_sites: 2
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCCCGCGAATCTGATCAATT	TCTGACTTTCGCGAGACATG	50.0	50.0	59.1	58.0	271-272 bp	1.0	72.0
rpl22-rps19_p2	GCCCGCGAATCTGATCAATT	CCTACTCTGACTTTCGCGAG	50.0	55.0	59.1	57.8	276-277 bp	1.0	70.7
rpl22-rps19_p3	GCCCGCGAATCTGATCAATT	CTCTGACTTTCGCGAGACAT	50.0	50.0	59.1	57.7	272-273 bp	1.0	70.0
rpl22-rps19_p4	GCCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	52.6	50.0	58.4	58.0	271-272 bp	1.0	69.4
rpl22-rps19_p5	GCCCGCGAATCTGATCAATT	CAAATTGGGAGAATTCGCGC	50.0	50.0	59.1	58.5	295-296 bp	1.0	68.5