Hotspot

ycf4-cemA

LSC · 103463-104304 · 842 bp

Back to Goudaea

Pi 0.00845

MarkerSeek score 53.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 7
pi: 0.00845
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GCCTATTTCTTGGGCGTACC	TGGGAAAGAAGGCAATGCTT	55.0	45.0	58.7	58.0	907-911 bp	1.0	76.2
ycf4-cemA_p2	GGCCTATTTCTTGGGCGTAC	TGGGAAAGAAGGCAATGCTT	55.0	45.0	58.7	58.0	908-912 bp	1.0	76.2
ycf4-cemA_p3	GCCTATTTCTTGGGCGTACC	ATGAGAGAGAGACCCACCAG	55.0	55.0	58.7	57.8	953-957 bp	1.0	74.9
ycf4-cemA_p4	GGCCTATTTCTTGGGCGTAC	ATGAGAGAGAGACCCACCAG	55.0	55.0	58.7	57.8	954-958 bp	1.0	74.9
ycf4-cemA_p5	GCCTATTTCTTGGGCGTACC	ATGGGAAAGAAGGCAATGCT	55.0	45.0	58.7	57.8	908-912 bp	1.0	74.4