Hotspot

ndhC-trnV-UAC

LSC · 93316-94143 · 828 bp

Back to Goudaea

Pi 0.00881

MarkerSeek score 67.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 7
pi: 0.00881
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	932-965 bp	1.0	81.5
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAACTCC	GAGCCCAATGCAACAAACAA	55.0	45.0	58.0	57.8	1019-1052 bp	1.0	80.9
ndhC-trnV-UAC_p3	CGGGGCTAAAACTCCTGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	927-960 bp	1.0	80.3
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCTGAAA	GAGCCCAATGCAACAAACAA	50.0	45.0	57.8	57.8	1014-1047 bp	1.0	79.7
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAACTCCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	931-964 bp	1.0	79.1