Hotspot

trnS-GGA-rps4

LSC · 87787-88062 · 276 bp

Back to Goudaea

Pi 0.0145

MarkerSeek score 60.80

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 276
variable_sites: 4
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	AGTAAATGGATCGGTTTGCA	50.0	40.0	58.1	55.3	382 bp	1.0	58.8
trnS-GGA-rps4_p2	AGGCTTTTGTTTACCGAGGG	AGTAAATGGATCGGTTTGCAA	50.0	38.1	58.1	56.1	382 bp	1.0	57.2
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	AGTAAATGGATCGGTTTGCA	55.0	40.0	58.4	55.3	434 bp	1.0	56.4
trnS-GGA-rps4_p4	GGCTTTTGTTTACCGAGGGT	AGTAAATGGATCGGTTTGCA	50.0	40.0	58.4	55.3	381 bp	1.0	56.4
trnS-GGA-rps4_p5	AGGCTTTTGTTTACCGAGGG	AGTAAATGGATCGGTTTGCAAA	50.0	36.4	58.1	56.9	382 bp	1.0	55.1