Hotspot

trnG-GCC-trnfM-CAU

LSC · 79161-79298 · 138 bp

Back to Goudaea

Pi 0.058

MarkerSeek score 82.20

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 8
pi: 0.058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	204 bp	1.0	82.4
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	205 bp	1.0	82.3
trnG-GCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	245 bp	1.0	81.5
trnG-GCC-trnfM-CAU_p4	GTGTGGACATATACGTGCGT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	281 bp	1.0	81.3
trnG-GCC-trnfM-CAU_p5	TGTGTGGACATATACGTGCG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	282 bp	1.0	81.3