Hotspot

trnC-GCA-petN

LSC · 70021-71038 · 1018 bp

Back to Goudaea

Pi 0.0832

MarkerSeek score 84.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1018
variable_sites: 83
pi: 0.0832
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	1060-1067 bp	1.0	82.8
trnC-GCA-petN_p2	ATTTTGGATCGGATTTGGCG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	1125-1132 bp	1.0	79.2
trnC-GCA-petN_p3	TTCAAATCTGGGTGTCGCC	AGCCCAAGCGAGACTTACTA	52.6	50.0	58.1	58.2	1058-1065 bp	1.0	78.2
trnC-GCA-petN_p4	AGTTCAAATCTGGGTGTCGC	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.5	57.1	1059-1066 bp	1.0	76.9
trnC-GCA-petN_p5	TTTTGGATCGGATTTGGCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	59.2	58.2	1124-1131 bp	1.0	76.7