Hotspot

ndhD-ccsA

SSC · 128281-128591 · 311 bp

Back to Gossypium

Pi 0.0319

MarkerSeek score 52.60

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 311
variable_sites: 23
pi: 0.0319
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	CTCATGGCTTTATCGGTGCT	TTGGACCGTATTCGCGATTT	50.0	45.0	58.0	58.0	968-1000 bp	1.0	83.0
ndhD-ccsA_p2	TCTCATGGCTTTATCGGTGC	TTGGACCGTATTCGCGATTT	50.0	45.0	58.0	58.0	969-1001 bp	1.0	83.0
ndhD-ccsA_p3	CTCATGGCTTTATCGGTGCT	GTAAATTCCGCACTTGTGGC	50.0	50.0	58.0	58.0	913-945 bp	1.0	83.0
ndhD-ccsA_p4	TCTCATGGCTTTATCGGTGC	GTAAATTCCGCACTTGTGGC	50.0	50.0	58.0	58.0	914-946 bp	1.0	82.9
ndhD-ccsA_p5	TATTACCCCATGCGCATTCC	TTGGACCGTATTCGCGATTT	50.0	45.0	58.1	58.0	1175-1207 bp	1.0	82.2