Hotspot

rpl33-rps18

LSC · 71912-72245 · 334 bp

Back to Gossypium

Pi 0.0189

MarkerSeek score 52.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 334
variable_sites: 24
pi: 0.0189
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AAAGGAGGTTCATGGCCAAG	GGGTTCCCTCGAGAATGAAC	50.0	55.0	58.1	58.0	1024-1114 bp	1.0	84.9
rpl33-rps18_p2	AAAGGAGGTTCATGGCCAAG	AATGCAGTAATTCGCGGAGT	50.0	45.0	58.1	58.0	1224-1314 bp	1.0	84.4
rpl33-rps18_p3	AATGTGGCTGGAACAACAGT	GGGTTCCCTCGAGAATGAAC	45.0	55.0	57.9	58.0	1288-1370 bp	0.941176	82.5
rpl33-rps18_p4	AATGTGGCTGGAACAACAGT	AATGCAGTAATTCGCGGAGT	45.0	45.0	57.9	58.0	1488-1570 bp	0.941176	82.1
rpl33-rps18_p5	AAAGGAGGTTCATGGCCAAG	CCCAACCGGATTCGATTCTT	50.0	50.0	58.1	58.0	886-986 bp	0.941176	79.6