Hotspot

psaJ-rpl33

LSC · 71216-71710 · 495 bp

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Pi 0.0165

MarkerSeek score 55.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 25
pi: 0.0165
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCCCGGATGCGTTAACATT	ATTAACGCCGTTTCGAACAC	45.0	45.0	57.9	57.4	597-630 bp	1.0	80.9
psaJ-rpl33_p2	TTCCCGGATGCGTTAACATT	TAACGCCGTTTCGAACACAA	45.0	45.0	57.9	58.7	595-628 bp	1.0	79.8
psaJ-rpl33_p3	TTCCCGGATGCGTTAACATT	TTAACGCCGTTTCGAACACA	45.0	45.0	57.9	58.7	596-629 bp	1.0	79.8
psaJ-rpl33_p4	GTTTCTTCCCGGATGCGTTA	ATTAACGCCGTTTCGAACAC	50.0	45.0	58.3	57.4	602-635 bp	1.0	79.6
psaJ-rpl33_p5	TTCTTCCCGGATGCGTTAAC	ATTAACGCCGTTTCGAACAC	50.0	45.0	58.3	57.4	600-633 bp	1.0	79.6