Hotspot

psbE-petL

LSC · 68959-69808 · 850 bp

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Pi 0.0219

MarkerSeek score 60.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 46
pi: 0.0219
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	57.8	57.3	927-1003 bp	1.0	62.1
psbE-petL_p2	AATAACCAACCCGCGATGAA	TGAGGTTATAGTTAAAGCCGCT	45.0	40.9	57.9	57.3	1010-1086 bp	1.0	62.0
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	TGAGGTTATAGTTAAAGCCGCT	55.0	40.9	58.2	57.3	922-998 bp	1.0	61.8
psbE-petL_p4	TGCTGACGAATAACCAACCC	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	58.2	57.3	1018-1094 bp	1.0	61.4
psbE-petL_p5	AACCAACCCGCGATGAATAG	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	58.3	57.3	1007-1083 bp	1.0	60.2