Hotspot

psaA-ycf3

LSC · 43862-44787 · 926 bp

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Pi 0.0145

MarkerSeek score 62.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 35
pi: 0.0145
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	1065-1124 bp	1.0	84.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1083-1142 bp	1.0	84.4
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	1085-1144 bp	1.0	83.6
psaA-ycf3_p4	CCTAGCCCATTCCTCGAAAG	ATCAAGCCGCTGAGTATTGG	55.0	50.0	57.8	58.0	1087-1146 bp	1.0	83.4
psaA-ycf3_p5	CCTAGCCCATTCCTCGAAAG	GGAAACAGGCTATAGCGCTT	55.0	50.0	57.8	58.0	1069-1128 bp	1.0	83.4