Hotspot

psbZ-trnG-GCC

LSC · 37734-38492 · 759 bp

Back to Gossypium

Pi 0.0248

MarkerSeek score 75.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 67
pi: 0.0248
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	792-937 bp	1.0	87.0
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	790-935 bp	1.0	87.0
psbZ-trnG-GCC_p3	CTCCTGATGGTTGGTTGAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	786-931 bp	1.0	81.8
psbZ-trnG-GCC_p4	TGCTTCTCCTGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.9	57.8	791-936 bp	1.0	81.2
psbZ-trnG-GCC_p5	TCTCCTGATGGTTGGTTGAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	787-932 bp	1.0	79.5