Hotspot

trnR-UCU-atpA

LSC · 10196-10241 · 46 bp

Back to Gossypium

Pi 0.0276

MarkerSeek score 61.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 46
variable_sites: 5
pi: 0.0276
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTCACTGAGGAAGCGGAA	47.6	50.0	57.3	57.9	146-151 bp	1.0	71.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCACTGAGGAAGCGGAAAC	47.6	50.0	57.3	58.1	144-149 bp	1.0	70.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTCACTGAGGAAGCG	47.6	50.0	57.3	57.6	149-154 bp	1.0	68.6
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	CATTCACTGAGGAAGCGGAA	47.6	50.0	56.9	57.9	181-186 bp	1.0	67.1
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	TTCACTGAGGAAGCGGAAAC	47.6	50.0	56.9	58.1	179-184 bp	1.0	67.0