Hotspot

trnS-GCU-trnG-GCC

LSC · 8189-9081 · 893 bp

Back to Gossypium

Pi 0.0131

MarkerSeek score 60.70

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 893
variable_sites: 38
pi: 0.0131
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	995-1031 bp	1.0	81.9
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	994-1030 bp	1.0	81.9
trnS-GCU-trnG-GCC_p3	CGCACAACGGATTAGCAATC	ATGAATCAAACCGAGGGACC	50.0	50.0	57.9	57.6	1010-1046 bp	1.0	81.1
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.2	57.6	1049-1085 bp	1.0	80.5
trnS-GCU-trnG-GCC_p5	TCGCACAACGGATTAGCAAT	ATGAATCAAACCGAGGGACC	45.0	50.0	58.3	57.6	1011-1047 bp	1.0	79.7