Hotspot

ycf1

SSC · 126553-132177 · 5625 bp

Back to Gordonia

Pi 0.0325

MarkerSeek score 59.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 289
pi: 0.0325
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCGCGCTTTCTCTTTTCT	ACGAAGGGGACTAAACAGGA	45.0	50.0	57.9	58.0	1568-1586 bp	1.0	85.7
ycf1_p2	TCCGCGCTTTCTCTTTTCTT	ACGAAGGGGACTAAACAGGA	45.0	50.0	58.1	58.0	1567-1585 bp	1.0	85.6
ycf1_p3	ATCCGCGCTTTCTCTTTTCT	ACAGGAACAAGAGGGATCCA	45.0	50.0	57.9	58.0	1554-1572 bp	1.0	85.6
ycf1_p4	ATCCGCGCTTTCTCTTTTCT	AAGAGGGATCCACCGAAGAA	45.0	50.0	57.9	58.0	1546-1564 bp	1.0	85.6
ycf1_p5	GCTACTTCTATCCGCGCTTT	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.1	58.0	1577-1595 bp	1.0	85.4