Hotspot

ndhF

SSC · 112898-115144 · 2247 bp

Back to Gordonia

Pi 0.0265

MarkerSeek score 59.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 97
pi: 0.0265
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	TGACCTATTGTGACTTCGTGA	50.0	42.9	58.0	56.7	2741-2753 bp	0.5	42.0
ndhF_p2	GCAGAATACCGTCACCCATT	TGACCTATTGTGACTTCGTGA	50.0	42.9	58.0	56.7	2830-2842 bp	0.5	41.9
ndhF_p3	ACAGGAACAAGAGGGATCCA	TGACCTATTGTGACTTCGTGA	50.0	42.9	58.0	56.7	2727-2739 bp	0.5	41.8
ndhF_p4	AAGAGGGATCCACCGAAGAA	TGACCTATTGTGACTTCGTGA	50.0	42.9	58.0	56.7	2719-2731 bp	0.5	41.8
ndhF_p5	GAGGATCCGGACAAAATCGA	ATCGAGGAAAGAATACGCCT	50.0	45.0	57.4	56.4	2990 bp	0.25	32.6