Hotspot

ndhG-ndhI

SSC · 121462-121836 · 375 bp

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Pi 0.0516

MarkerSeek score 76.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 375
variable_sites: 30
pi: 0.0516
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCTACAGCGATTGCACCTAT	AACAAACAGTACGAGCTGCA	50.0	45.0	58.1	58.1	1350-1363 bp	1.0	82.9
ndhG-ndhI_p2	GCTACAGCGATTGCACCTAT	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.1	58.1	1349-1362 bp	1.0	82.9
ndhG-ndhI_p3	GCTACAGCGATTGCACCTAT	CAAGGCTGAGAATGGACCAA	50.0	50.0	58.1	57.8	1588-1601 bp	1.0	81.4
ndhG-ndhI_p4	GCTACAGCGATTGCACCTAT	AAGGCTGAGAATGGACCAAC	50.0	50.0	58.1	57.8	1599-1600 bp	0.5	57.6
ndhG-ndhI_p5	GCTACAGCGATTGCACCTAT	TTTGTGGCAATTGCGTTGAA	50.0	40.0	58.1	57.7	1083-1084 bp	0.5	56.9