Hotspot

ndhF-rpl32

SSC · 115145-115815 · 671 bp

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Pi 0.0383

MarkerSeek score 73.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 671
variable_sites: 40
pi: 0.0383
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGCCCACATACGACGAA	TAGATTGGGTTGAAAGCGGG	45.0	50.0	57.8	57.9	1468-1629 bp	1.0	83.1
ndhF-rpl32_p2	CAGGCCGTGTAAACCAAAAC	TAGATTGGGTTGAAAGCGGG	50.0	50.0	57.9	57.9	1850-2011 bp	1.0	82.7
ndhF-rpl32_p3	ACAGGCCGTGTAAACCAAAA	TAGATTGGGTTGAAAGCGGG	45.0	50.0	58.2	57.9	1851-2012 bp	1.0	81.9
ndhF-rpl32_p4	AAAAGAAGAAGTCCCGCTCC	TAGATTGGGTTGAAAGCGGG	50.0	50.0	57.8	57.9	1428 bp	0.5	58.2
ndhF-rpl32_p5	AAGAAGAAGTCCCGCTCCTA	TAGATTGGGTTGAAAGCGGG	50.0	50.0	57.8	57.9	1426 bp	0.5	57.8