Hotspot

petA-psbJ

LSC · 65331-66379 · 1049 bp

Back to Gordonia

Pi 0.0285

MarkerSeek score 79.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1049
variable_sites: 46
pi: 0.0285
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1180-1192 bp	1.0	80.4
petA-psbJ_p2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1181-1193 bp	1.0	80.3
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.8	57.6	1147-1159 bp	1.0	80.2
petA-psbJ_p4	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1148-1160 bp	1.0	77.8
petA-psbJ_p5	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	1175-1187 bp	1.0	77.6