Hotspot

cemA-petA

LSC · 64134-64367 · 234 bp

Back to Gordonia

Pi 0.0328

MarkerSeek score 72.50

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 12
pi: 0.0328
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	AAATCGCGTATCTCCGTCAC	GCGATACGGAAATGGATCGA	50.0	50.0	58.2	57.9	341-355 bp	1.0	85.0
cemA-petA_p2	AAATCGCGTATCTCCGTCAC	AGCGATACGGAAATGGATCG	50.0	50.0	58.2	57.9	342-356 bp	1.0	84.9
cemA-petA_p3	AAATCGCGTATCTCCGTCAC	GCACTTGAAATGGATGTCCG	50.0	50.0	58.2	57.5	381-395 bp	1.0	80.7
cemA-petA_p4	GCGTATCTCCGTCACTTGTA	GCGATACGGAAATGGATCGA	50.0	50.0	57.2	57.9	336-350 bp	1.0	79.6
cemA-petA_p5	GCGTATCTCCGTCACTTGTA	AGCGATACGGAAATGGATCG	50.0	50.0	57.2	57.9	337-351 bp	1.0	79.6