primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAAATGAAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	7.8	0.0	0.089038	6179	7792	1762-1857	1.000000	87.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAAGGGCGTTTCCTTGTTCT	GAAATGAAAATGGGGCGTGG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.076117	6179	7792	2230-2315	1.000000	87.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TTGAGCGGTCTTTAAACCCC	GAAATGAAAATGGGGCGTGG	20	20	50.0	50.0	58.1	58.0	16.6	0.0	0.108506	6179	7792	2320-2405	1.000000	87.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAAGGGCGTTTCCTTGTTCT	CGAAAACTTACGGCAGCTTG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.116894	6179	7792	2735-2820	1.000000	85.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CTACTCGAGCTTCATCGTGG	GAAATGAAAATGGGGCGTGG	20	20	55.0	50.0	57.9	58.0	13.6	0.0	0.121226	6179	7792	1895-1912	0.500000	59.5
trnG-trnR-UCU_p1	trnG-trnR-UCU	1	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.4	52.4	0.0	0.0	7.001259	10563	10819	314-335	1.000000	48.4
trnG-trnR-UCU_p2	trnG-trnR-UCU	2	TAACGATGCGGGTTCGATTC	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.4	52.4	0.0	0.0	7.005652	10563	10819	313-334	1.000000	48.4
trnG-trnR-UCU_p3	trnG-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.0	52.4	0.0	0.0	6.598602	10563	10819	325-346	1.000000	48.3
trnG-trnR-UCU_p4	trnG-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	TGTCCTATCCATTAGACAATG	20	21	55.0	38.1	58.1	52.4	0.0	0.0	6.627438	10563	10819	328-349	1.000000	48.3
trnG-trnR-UCU_p5	trnG-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	TGTCCTATCCATTAGACAATG	20	21	55.0	38.1	57.7	52.4	0.0	0.0	6.902452	10563	10819	329-350	1.000000	48.2
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	GGACTGGTTGTTGCATTAGC	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.755081	13859	14227	486-528	1.000000	78.2
atpF-atpH_p2	atpF-atpH	2	CCAGTAGCCCAAAGAAACGA	GGACTGGTTGTTGCATTAGC	20	20	50.0	50.0	57.8	57.4	0.0	0.0	0.815680	13859	14227	459-501	1.000000	78.0
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.804163	13859	14227	551-593	1.000000	77.3
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.821586	13859	14227	552-594	1.000000	77.2
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.821586	13859	14227	553-595	1.000000	77.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CTTCATTTCCATCCCGTAGCA	AAAAGGATTTGCAGTCCCCC	21	20	47.6	50.0	58.1	58.4	0.0	14.6	1.437558	27817	29058	1279-1308	1.000000	75.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTCATTTCCATCCCGTAGCA	AAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	56.9	58.4	0.0	14.6	1.446590	27817	29058	1278-1307	1.000000	75.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TTCCTTCATTTCCATCCCGT	AAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	56.8	58.4	0.0	14.6	1.549445	27817	29058	1282-1311	1.000000	74.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCATTTCCATCCCGTAGCAT	AAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	56.7	58.4	0.0	14.6	1.678353	27817	29058	1277-1306	1.000000	73.7
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TTCATTTCCATCCCGTAGCAT	AAAAGGATTTGCAGTCCCCC	21	20	42.9	50.0	57.4	58.4	0.0	14.6	1.931569	27817	29058	1278-1307	1.000000	71.7
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	32361	33164	916-971	1.000000	85.5
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	32361	33164	895-950	1.000000	85.2
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	32361	33164	892-947	1.000000	85.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32361	33164	878-933	1.000000	85.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32361	33164	857-912	1.000000	85.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.3	53.0	0.0	6.7	5.273069	33237	34755	1565-1590	1.000000	46.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	20	20	55.0	40.0	58.1	53.0	7.6	6.7	5.054330	33237	34755	1588-1613	1.000000	46.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTATAGTCAT	20	20	55.0	40.0	58.1	52.8	7.6	6.7	5.305690	33237	34755	1587-1612	1.000000	46.8
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.1	53.0	0.0	6.7	5.076485	33237	34755	1591-1616	1.000000	46.7
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCCCTTTTAACTCAGTGGT	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	57.7	53.0	0.0	6.7	5.278240	33237	34755	1603-1628	1.000000	46.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	TCTTTGGAAAAGGGGGAACG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.2	0.0	0.014917	48684	49686	1172-1184	1.000000	91.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TCTTTGGAAAAGGGGGAACG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.0	58.1	0.2	0.0	0.079726	48684	49686	1180-1192	1.000000	90.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	CCTGTTGATCCCTTGGCTTT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.075756	48684	49686	1656-1669	1.000000	88.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	TTAACTAATTGGCGGGCTCC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	2.4	0.0	0.051626	48684	49686	1752-1765	1.000000	88.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CGAAGCCATACCCAATCGAA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.056519	48684	49686	1723-1736	1.000000	88.4
cemA-petA_p1	cemA-petA	1	AAATCGCGTATCTCCGTCAC	GCGATACGGAAATGGATCGA	20	20	50.0	50.0	58.2	57.9	5.8	12.2	0.223942	64134	64367	341-355	1.000000	85.0
cemA-petA_p2	cemA-petA	2	AAATCGCGTATCTCCGTCAC	AGCGATACGGAAATGGATCG	20	20	50.0	50.0	58.2	57.9	5.8	4.1	0.229609	64134	64367	342-356	1.000000	84.9
cemA-petA_p3	cemA-petA	3	AAATCGCGTATCTCCGTCAC	GCACTTGAAATGGATGTCCG	20	20	50.0	50.0	58.2	57.5	5.8	0.0	0.713250	64134	64367	381-395	1.000000	80.7
cemA-petA_p4	cemA-petA	4	GCGTATCTCCGTCACTTGTA	GCGATACGGAAATGGATCGA	20	20	50.0	50.0	57.2	57.9	0.0	12.2	0.898712	64134	64367	336-350	1.000000	79.6
cemA-petA_p5	cemA-petA	5	GCGTATCTCCGTCACTTGTA	AGCGATACGGAAATGGATCG	20	20	50.0	50.0	57.2	57.9	0.0	4.1	0.904379	64134	64367	337-351	1.000000	79.6
petA-psbJ_p1	petA-psbJ	1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	0.0	4.1	0.543756	65331	66379	1180-1192	1.000000	80.4
petA-psbJ_p2	petA-psbJ	2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	0.0	4.1	0.548898	65331	66379	1181-1193	1.000000	80.3
petA-psbJ_p3	petA-psbJ	3	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.8	57.6	0.0	4.1	0.586979	65331	66379	1147-1159	1.000000	80.2
petA-psbJ_p4	petA-psbJ	4	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.5	57.6	0.0	4.1	0.879177	65331	66379	1148-1160	1.000000	77.8
petA-psbJ_p5	petA-psbJ	5	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.5	57.6	17.6	4.1	0.894921	65331	66379	1175-1187	1.000000	77.6
psbT-psbN_p1	psbT-psbN	1	TCGAGAACCGCCTAAAGTTC	TCGAGGAACATGGGGACTAA	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.600220	76841	76900	120-120	1.000000	77.9
psbT-psbN_p2	psbT-psbN	2	TCGAGAACCGCCTAAAGTTC	TTCGAGGAACATGGGGACTA	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.600220	76841	76900	121-121	1.000000	77.8
psbT-psbN_p3	psbT-psbN	3	TCGAGAACCGCCTAAAGTTC	CATTCGAGGAACATGGGGAC	20	20	50.0	55.0	57.6	58.3	0.0	0.0	0.690520	76841	76900	123-123	1.000000	77.1
psbT-psbN_p4	psbT-psbN	4	GAGAACCGCCTAAAGTTCCA	TCGAGGAACATGGGGACTAA	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.708847	76841	76900	118-118	1.000000	77.0
psbT-psbN_p5	psbT-psbN	5	TCGAGAACCGCCTAAAGTTC	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.6	57.7	0.0	0.0	0.643202	76841	76900	175-175	1.000000	76.7
rps8-rpl14_p1	rps8-rpl14	1	AGCAATAGTGTCCCTACCCA	GCCCGAGAATTGAGACAGTT	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.364500	83133	83321	273-280	1.000000	82.3
rps8-rpl14_p2	rps8-rpl14	2	AGCAATAGTGTCCCTACCCA	TCATTGGCTCCCGAGGTATT	20	20	50.0	50.0	57.7	58.5	0.0	0.0	0.756755	83133	83321	234-241	1.000000	80.0
rps8-rpl14_p3	rps8-rpl14	3	AGCAATAGTGTCCCTACCCA	TTCATTGGCTCCCGAGGTAT	20	20	50.0	50.0	57.7	58.5	0.0	0.0	0.756755	83133	83321	235-242	1.000000	80.0
rps8-rpl14_p4	rps8-rpl14	4	CAGCAATAGTGTCCCTACCC	GCCCGAGAATTGAGACAGTT	20	20	55.0	50.0	57.4	57.9	0.0	0.0	0.722668	83133	83321	274-281	1.000000	79.4
rps8-rpl14_p5	rps8-rpl14	5	AGCAATAGTGTCCCTACCCA	TAGTTTCATTGGCTCCCGAG	20	20	50.0	50.0	57.7	57.3	0.0	0.0	0.955304	83133	83321	239-246	1.000000	78.3
ndhF_p1	ndhF	1	ACGAAGGGGACTAAACAGGA	TGACCTATTGTGACTTCGTGA	20	21	50.0	42.9	58.0	56.7	0.0	0.0	2.317332	112898	115144	2741-2753	0.500000	42.0
ndhF_p2	ndhF	2	GCAGAATACCGTCACCCATT	TGACCTATTGTGACTTCGTGA	20	21	50.0	42.9	58.0	56.7	0.0	0.0	2.335912	112898	115144	2830-2842	0.500000	41.9
ndhF_p3	ndhF	3	ACAGGAACAAGAGGGATCCA	TGACCTATTGTGACTTCGTGA	20	21	50.0	42.9	58.0	56.7	15.2	0.0	2.342318	112898	115144	2727-2739	0.500000	41.8
ndhF_p4	ndhF	4	AAGAGGGATCCACCGAAGAA	TGACCTATTGTGACTTCGTGA	20	21	50.0	42.9	58.0	56.7	15.2	0.0	2.344904	112898	115144	2719-2731	0.500000	41.8
ndhF_p5	ndhF	5	GAGGATCCGGACAAAATCGA	ATCGAGGAAAGAATACGCCT	20	20	50.0	45.0	57.4	56.4	6.1	0.0	2.168845	112898	115144	2990-2990	0.250000	32.6
ndhF-rpl32_p1	ndhF-rpl32	1	AAAAGCCCACATACGACGAA	TAGATTGGGTTGAAAGCGGG	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.295634	115145	115815	1468-1629	1.000000	83.1
ndhF-rpl32_p2	ndhF-rpl32	2	CAGGCCGTGTAAACCAAAAC	TAGATTGGGTTGAAAGCGGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.251151	115145	115815	1850-2011	1.000000	82.7
ndhF-rpl32_p3	ndhF-rpl32	3	ACAGGCCGTGTAAACCAAAA	TAGATTGGGTTGAAAGCGGG	20	20	45.0	50.0	58.2	57.9	6.1	0.0	0.357553	115145	115815	1851-2012	1.000000	81.9
ndhF-rpl32_p4	ndhF-rpl32	4	AAAAGAAGAAGTCCCGCTCC	TAGATTGGGTTGAAAGCGGG	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.309315	115145	115815	1428-1428	0.500000	58.2
ndhF-rpl32_p5	ndhF-rpl32	5	AAGAAGAAGTCCCGCTCCTA	TAGATTGGGTTGAAAGCGGG	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.356088	115145	115815	1426-1426	0.500000	57.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGAAATCTCTTTCAAGCGG	GACGGAATGGAAATCGGGAA	20	20	50.0	50.0	56.8	58.0	1.0	0.0	1.246254	115978	116898	1126-1126	0.250000	40.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGAAATCTCTTTCAAGCGG	ATGACGGAATGGAAATCGGG	20	20	50.0	50.0	56.8	58.0	1.0	0.0	1.246255	115978	116898	1128-1128	0.250000	40.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGAAATCTCTTTCAAGCGG	GACGGAATGGAAATCGGGAA	21	20	47.6	50.0	58.3	58.0	1.0	0.0	1.316463	115978	116898	1127-1127	0.250000	39.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGAAATCTCTTTCAAGCGG	ATGACGGAATGGAAATCGGG	21	20	47.6	50.0	58.3	58.0	1.0	0.0	1.316464	115978	116898	1129-1129	0.250000	39.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGAAATCTCTTTCAAGCGGA	GACGGAATGGAAATCGGGAA	21	20	47.6	50.0	58.3	58.0	1.0	0.0	1.321138	115978	116898	1126-1126	0.250000	39.4
ndhG-ndhI_p1	ndhG-ndhI	1	GCTACAGCGATTGCACCTAT	AACAAACAGTACGAGCTGCA	20	20	50.0	45.0	58.1	58.1	7.9	1.0	0.180816	121462	121836	1350-1363	1.000000	82.9
ndhG-ndhI_p2	ndhG-ndhI	2	GCTACAGCGATTGCACCTAT	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	58.1	58.1	7.9	1.0	0.180816	121462	121836	1349-1362	1.000000	82.9
ndhG-ndhI_p3	ndhG-ndhI	3	GCTACAGCGATTGCACCTAT	CAAGGCTGAGAATGGACCAA	20	20	50.0	50.0	58.1	57.8	7.9	0.0	0.333942	121462	121836	1588-1601	1.000000	81.4
ndhG-ndhI_p4	ndhG-ndhI	4	GCTACAGCGATTGCACCTAT	AAGGCTGAGAATGGACCAAC	20	20	50.0	50.0	58.1	57.8	7.9	0.0	0.335496	121462	121836	1599-1600	0.500000	57.6
ndhG-ndhI_p5	ndhG-ndhI	5	GCTACAGCGATTGCACCTAT	TTTGTGGCAATTGCGTTGAA	20	20	50.0	40.0	58.1	57.7	7.9	23.5	0.428913	121462	121836	1083-1084	0.500000	56.9
ycf1_p1	ycf1	1	ATCCGCGCTTTCTCTTTTCT	ACGAAGGGGACTAAACAGGA	20	20	45.0	50.0	57.9	58.0	5.6	0.0	0.120412	130525	130644	1568-1586	1.000000	85.7
ycf1_p2	ycf1	2	TCCGCGCTTTCTCTTTTCTT	ACGAAGGGGACTAAACAGGA	20	20	45.0	50.0	58.1	58.0	5.6	0.0	0.140854	130525	130644	1567-1585	1.000000	85.6
ycf1_p3	ycf1	3	ATCCGCGCTTTCTCTTTTCT	ACAGGAACAAGAGGGATCCA	20	20	45.0	50.0	57.9	58.0	5.6	15.2	0.145397	130525	130644	1554-1572	1.000000	85.6
ycf1_p4	ycf1	4	ATCCGCGCTTTCTCTTTTCT	AAGAGGGATCCACCGAAGAA	20	20	45.0	50.0	57.9	58.0	5.6	15.2	0.147983	130525	130644	1546-1564	1.000000	85.6
ycf1_p5	ycf1	5	GCTACTTCTATCCGCGCTTT	ACGAAGGGGACTAAACAGGA	20	20	50.0	50.0	58.1	58.0	5.6	0.0	0.165581	130525	130644	1577-1595	1.000000	85.4