Hotspot

rpl36-infA

LSC · 79056-79176 · 121 bp

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Pi 0.0479

MarkerSeek score 82.70

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 20
pi: 0.0479
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	CCTACGAATCAGGCGACATT	CAACCAGGGGACGCATAATT	50.0	50.0	57.8	58.2	230-244 bp	1.0	84.5
rpl36-infA_p2	CCTACGAATCAGGCGACATT	AACCAGGGGACGCATAATTT	50.0	45.0	57.8	57.5	229-243 bp	1.0	82.2
rpl36-infA_p3	ATAATTCGTCCTCGCCTACG	CAACCAGGGGACGCATAATT	50.0	50.0	57.3	58.2	244-258 bp	1.0	80.6
rpl36-infA_p4	TTTTACGAACAGAGGCCCTT	CAACCAGGGGACGCATAATT	45.0	50.0	57.1	58.2	201-215 bp	1.0	79.9
rpl36-infA_p5	CCTACGAATCAGGCGACATT	TATGATTCAACCAGGGGACG	50.0	50.0	57.8	57.0	237-251 bp	1.0	78.2