Hotspot

clpP-psbB

LSC · 70992-71638 · 647 bp

Back to Goodyera

Pi 0.0647

MarkerSeek score 86.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 647
variable_sites: 119
pi: 0.0647
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGGGGAAATACCGTATGACC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.8	58.2	650-796 bp	1.0	88.8
clpP-psbB_p2	CGGGGAAATACCGTATGACC	CAATCGACCGGGATCATTCA	55.0	50.0	57.8	57.8	687-833 bp	1.0	88.8
clpP-psbB_p3	TCCAGGACTCCGAAAAGGTA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	580-726 bp	1.0	87.6
clpP-psbB_p4	TCCAGGACTCCGAAAAGGTA	CAATCGACCGGGATCATTCA	50.0	50.0	57.7	57.8	617-763 bp	1.0	87.6
clpP-psbB_p5	CCAGGACTCCGAAAAGGTAC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.6	58.2	579-725 bp	1.0	87.0