Hotspot

rps18-rpl20

LSC · 67263-67525 · 263 bp

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Pi 0.0598

MarkerSeek score 82.20

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 263
variable_sites: 59
pi: 0.0598
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	ATTGGATAGAGCGGAGTCGA	AACGAGGAAACCCGCTATTC	50.0	50.0	58.0	58.0	881-944 bp	1.0	91.7
rps18-rpl20_p2	TTGGATAGAGCGGAGTCGAT	AACGAGGAAACCCGCTATTC	50.0	50.0	58.0	58.0	880-943 bp	1.0	91.7
rps18-rpl20_p3	ATTGGATAGAGCGGAGTCGA	GAAACGAGGAAACCCGCTAT	50.0	50.0	58.0	58.0	883-946 bp	0.818182	84.4
rps18-rpl20_p4	TTGGATAGAGCGGAGTCGAT	ATAGAAACGAGGAAACCCGC	50.0	50.0	58.0	58.0	885-948 bp	0.818182	84.4
rps18-rpl20_p5	TTGGATAGAGCGGAGTCGAT	GAAACGAGGAAACCCGCTAT	50.0	50.0	58.0	58.0	882-945 bp	0.818182	84.4