Hotspot

petA-psbJ

LSC · 62407-63361 · 955 bp

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Pi 0.0461

MarkerSeek score 83.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 139
pi: 0.0461
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCATCAGATGGACGTCAAG	CACAGAAAACCGACAAGGGA	50.0	50.0	58.0	58.0	1778-1830 bp	1.0	90.8
petA-psbJ_p2	TGCATCAGATGGACGTCAAG	TCACAGAAAACCGACAAGGG	50.0	50.0	58.0	58.0	1779-1831 bp	1.0	90.8
petA-psbJ_p3	GCATCAGATGGACGTCAAGT	CACAGAAAACCGACAAGGGA	50.0	50.0	58.0	58.0	1777-1829 bp	1.0	90.8
petA-psbJ_p4	TGCATCAGATGGACGTCAAG	TTTGTCTCTAGCATGACCGC	50.0	50.0	58.0	58.0	1351-1399 bp	0.636364	75.3
petA-psbJ_p5	GCATCAGATGGACGTCAAGT	TTTGTCTCTAGCATGACCGC	50.0	50.0	58.0	58.0	1350-1398 bp	0.636364	75.3