Hotspot

trnE-UUC-trnT-GGU

LSC · 30546-30956 · 411 bp

Back to Goodyera

Pi 0.0408

MarkerSeek score 82.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 59
pi: 0.0408
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	360-528 bp	1.0	90.6
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	339-507 bp	1.0	90.1
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	336-504 bp	1.0	90.0
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	338-506 bp	1.0	89.7
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	TGGAAAAAGCCCCTTATCGG	50.0	50.0	57.8	57.9	384-552 bp	1.0	89.5