Hotspot

trnS-GCU-trnG-UCC

LSC · 7983-8469 · 487 bp

Back to Goodyera

Pi 0.038

MarkerSeek score 81.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 487
variable_sites: 68
pi: 0.038
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	406-599 bp	1.0	63.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	405-598 bp	1.0	63.7
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	408-601 bp	1.0	62.6
trnS-GCU-trnG-UCC_p4	GTTATCAGGGGAGTCGGAGA	ACGAATCACACTTTTACCACT	55.0	38.1	57.9	55.5	473-666 bp	0.909091	59.5
trnS-GCU-trnG-UCC_p5	AGTTATCAGGGGAGTCGGAG	ACGAATCACACTTTTACCACT	55.0	38.1	57.9	55.5	474-667 bp	0.909091	59.5