Hotspot

trnQ-UUG-psbK

LSC · 6611-6995 · 385 bp

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Pi 0.0497

MarkerSeek score 81.20

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 60
pi: 0.0497
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GACCAAAACCCGTTGCCTTA	AGGGTAGAATTAAGACAGATACAGA	50.0	36.0	58.7	56.5	405-470 bp	1.0	52.0
trnQ-UUG-psbK_p2	CGAATAACGGGACCAAAACC	AGGGTAGAATTAAGACAGATACAGA	50.0	36.0	57.1	56.5	415-480 bp	1.0	52.0
trnQ-UUG-psbK_p3	ACCAAAACCCGTTGCCTTAC	AGGGTAGAATTAAGACAGATACAGA	50.0	36.0	59.0	56.5	404-469 bp	1.0	52.0
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	GGGTAGAATTAAGACAGATACAGA	50.0	37.5	58.7	55.2	404-469 bp	1.0	52.0
trnQ-UUG-psbK_p5	GACCAAAACCCGTTGCCTTA	AGGGTAGAATTAAGACAGATACAG	50.0	37.5	58.7	55.2	405-470 bp	1.0	52.0