Hotspot

rps16-trnQ-UUG

LSC · 6101-6538 · 438 bp

Back to Goodyera

Pi 0.056

MarkerSeek score 74.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 438
variable_sites: 71
pi: 0.056
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	335-693 bp	1.0	89.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	402-682 bp	0.909091	86.6
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	AAAAGAAAAATGGGGCGTGG	50.0	45.0	58.2	57.5	393-751 bp	0.909091	82.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	406-686 bp	0.909091	81.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AAAAGAAAAATGGGGCGTGG	45.0	45.0	58.1	57.5	460-740 bp	0.818182	79.3