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trnK-UUU-rps16

LSC · 4333-4890 · 558 bp

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Pi 0.0454

MarkerSeek score 80.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 89
pi: 0.0454
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1233-1292 bp	0.909091	86.7
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1232-1291 bp	0.909091	86.7
trnK-UUU-rps16_p3	CTTTTCCCACATCAGGCACT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.1	58.0	1624-1679 bp	0.818182	83.0
trnK-UUU-rps16_p4	CTTTTCCCACATCAGGCACT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	1209-1273 bp	0.818182	82.8
trnK-UUU-rps16_p5	CTTTTCCCACATCAGGCACT	GTGCAAAGCAAAGACAACGA	50.0	45.0	58.1	57.8	1631-1686 bp	0.636364	72.9