Hotspot

rps16

LSC · 5056-6224 · 1169 bp

Back to Gonystylus

Pi 0.000861

MarkerSeek score 55.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1169
variable_sites: 1
pi: 0.000861
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAGTCATCCACACCCTCTT	TGTACCGACTGAACCAATGAC	50.0	47.6	58.0	58.0	1299-1307 bp	1.0	72.0
rps16_p2	AAAGGAAAAGAGGGGGAGGA	TGTACCGACTGAACCAATGAC	50.0	47.6	57.9	58.0	1352-1360 bp	1.0	71.1
rps16_p3	AAGGAAAAGAGGGGGAGGAA	TGTACCGACTGAACCAATGAC	50.0	47.6	57.9	58.0	1351-1359 bp	1.0	71.1
rps16_p4	AGGAAAAGAGGGGGAGGAAA	TGTACCGACTGAACCAATGAC	50.0	47.6	57.9	58.0	1350-1358 bp	1.0	71.1
rps16_p5	ATGAGTCATCCACACCCTCT	TGTACCGACTGAACCAATGAC	50.0	47.6	57.7	58.0	1300-1308 bp	1.0	70.2