Hotspot

rpoA

LSC · 82576-83559 · 984 bp

Back to Gonystylus

Pi 0.00305

MarkerSeek score 53.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 984
variable_sites: 3
pi: 0.00305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGGATTGGATTGTGCTGAAGA	TGGCTGTAGACCCCCTAAAA	42.9	50.0	57.6	58.0	1157 bp	1.0	68.3
rpoA_p2	TGGATTGGATTGTGCTGAAGA	AATGGCTGTAGACCCCCTAA	42.9	50.0	57.6	57.7	1159 bp	1.0	66.5
rpoA_p3	TGGATTGGATTGTGCTGAAGA	ATGGCTGTAGACCCCCTAAA	42.9	50.0	57.6	57.7	1158 bp	1.0	66.5
rpoA_p4	TGGATTGGATTGTGCTGAAGA	ATAATGGCTGTAGACCCCCT	42.9	50.0	57.6	57.5	1161 bp	1.0	64.6
rpoA_p5	TGGATTGGATTGTGCTGAAGA	CATAATGGCTGTAGACCCCC	42.9	55.0	57.6	57.4	1162 bp	1.0	64.2