Hotspot

rps15-ycf1

IRa · 145149-145571 · 423 bp

Back to Gonystylus

Pi 0.0118

MarkerSeek score 61.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: IRa
length_bp: 423
variable_sites: 5
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGAAATTCAACAGACCCCCG	TGGCAGTCGTTTCAGTATGG	50.0	50.0	58.1	57.9	549 bp	1.0	79.2
rps15-ycf1_p2	TGAAATTCAACAGACCCCCG	GGCAGTCGTTTCAGTATGGT	50.0	50.0	58.1	57.9	548 bp	1.0	79.2
rps15-ycf1_p3	TGAAATTCAACAGACCCCCG	TGGCCCAATCATCGACTAGA	50.0	50.0	58.1	58.2	611 bp	1.0	78.2
rps15-ycf1_p4	TGAAATTCAACAGACCCCCG	CTTTGGCCCAATCATCGACT	50.0	50.0	58.1	58.2	614 bp	1.0	77.9
rps15-ycf1_p5	TGAAATTCAACAGACCCCCG	TCTTTGGCCCAATCATCGAC	50.0	50.0	58.1	58.2	615 bp	1.0	77.9