Hotspot

ycf1-rps15

IRb · 119928-120350 · 423 bp

Back to Gonystylus

Pi 0.0118

MarkerSeek score 61.90

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: IRb
length_bp: 423
variable_sites: 5
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TGGCAGTCGTTTCAGTATGG	TGAAATTCAACAGACCCCCG	50.0	50.0	57.9	58.1	549 bp	1.0	79.2
ycf1-rps15_p2	GGCAGTCGTTTCAGTATGGT	TGAAATTCAACAGACCCCCG	50.0	50.0	57.9	58.1	548 bp	1.0	79.2
ycf1-rps15_p3	TGGCCCAATCATCGACTAGA	TGAAATTCAACAGACCCCCG	50.0	50.0	58.2	58.1	611 bp	1.0	78.2
ycf1-rps15_p4	CTTTGGCCCAATCATCGACT	TGAAATTCAACAGACCCCCG	50.0	50.0	58.2	58.1	614 bp	1.0	77.9
ycf1-rps15_p5	TCTTTGGCCCAATCATCGAC	TGAAATTCAACAGACCCCCG	50.0	50.0	58.2	58.1	615 bp	1.0	77.9