Hotspot

rps7-rps12

IRb · 102706-103337 · 632 bp

Back to Gonystylus

Pi 0

MarkerSeek score 63.60

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 632
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TTTTTCTGCAGTACCTCGGC	CCCTAGATGCTGTCGGAGTA	50.0	55.0	58.5	58.0	683-715 bp	1.0	79.7
rps7-rps12_p2	TTTTTCTGCAGTACCTCGGC	AGGATTTACCCGGTGTGAGA	50.0	50.0	58.5	58.1	722-754 bp	1.0	79.2
rps7-rps12_p3	TTTTTCTGCAGTACCTCGGC	GAGGGGGAAGGGTTAAGGAT	50.0	55.0	58.5	58.1	737-769 bp	1.0	78.8
rps7-rps12_p4	TTTTTCTGCAGTACCTCGGC	GCTGTCGGAGTAAAGGATCG	50.0	55.0	58.5	58.2	675-707 bp	1.0	78.6
rps7-rps12_p5	TTTTTCTGCAGTACCTCGGC	ACCCGGTGTGAGATATCACA	50.0	50.0	58.5	58.1	715-747 bp	1.0	78.5