Hotspot

petD-rpoA

LSC · 82377-82575 · 199 bp

Back to Gonystylus

Pi 0.0503

MarkerSeek score 79.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 10
pi: 0.0503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CAGTAGCGACAACCGTCTTT	AGAGCCAGGAAGACCTTATGA	50.0	47.6	58.2	57.9	386 bp	1.0	71.3
petD-rpoA_p2	AGCGACAACCGTCTTTTTGA	AGAGCCAGGAAGACCTTATGA	45.0	47.6	58.3	57.9	382 bp	1.0	70.4
petD-rpoA_p3	TTGATTGGTACTGCAGTGGC	AGAGCCAGGAAGACCTTATGA	50.0	47.6	58.5	57.9	366 bp	1.0	69.5
petD-rpoA_p4	AGTAGCGACAACCGTCTTTT	AGAGCCAGGAAGACCTTATGA	45.0	47.6	57.5	57.9	385 bp	1.0	68.9
petD-rpoA_p5	TTGGGCATTGGAGCAACATT	AGAGCCAGGAAGACCTTATGA	45.0	47.6	58.6	57.9	339 bp	1.0	68.2