Hotspot

petN-psbM

LSC · 29979-31224 · 1246 bp

Back to Gonystylus

Pi 0.0169

MarkerSeek score 68.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1246
variable_sites: 21
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	GCTACTGCGCTCTTCATTCT	50.0	50.0	58.2	58.1	1407-1470 bp	1.0	79.6
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGCTCTTCATT	50.0	45.0	58.2	57.9	1409-1472 bp	1.0	79.4
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	GCTACTGCGCTCTTCATTCT	50.0	50.0	57.6	58.1	1346-1409 bp	1.0	78.1
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TTGCTACTGCGCTCTTCATT	50.0	45.0	57.6	57.9	1348-1411 bp	1.0	77.8
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTCTTCAT	50.0	45.0	58.2	57.7	1410-1473 bp	1.0	77.6