Hotspot

rps2-rpoC2

LSC · 17260-17653 · 394 bp

Back to Gonystylus

Pi 0.0136
MarkerSeek score 58.10
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
394
variable_sites
5
pi
0.0136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 ACCAAAATGAACTCCCGCTT GTACCCTGTTCAAGGCAACA 45.0 50.0 58.0 58.0 548-559 bp 1.0 84.2
rps2-rpoC2_p2 GCTTCCAGCATCTCTTCCAA GTACCCTGTTCAAGGCAACA 50.0 50.0 57.9 58.0 532-543 bp 1.0 83.5
rps2-rpoC2_p3 AAATGAACTCCCGCTTCCAG GTACCCTGTTCAAGGCAACA 50.0 50.0 58.2 58.0 544-555 bp 1.0 82.9
rps2-rpoC2_p4 ACCAAAATGAACTCCCGCTT AGTACCCTGTTCAAGGCAAC 45.0 50.0 58.0 57.7 549-560 bp 1.0 82.3
rps2-rpoC2_p5 AAAATGAACTCCCGCTTCCA GTACCCTGTTCAAGGCAACA 45.0 50.0 57.7 58.0 545-556 bp 1.0 82.0