Hotspot

atpF-atpH

LSC · 13595-14156 · 562 bp

Back to Gonystylus

Pi 0.00374

MarkerSeek score 64.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 2
pi: 0.00374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAGAAAACGA	TGGTCTGGTTGTAGCATTGG	50.0	50.0	58.0	57.8	627-653 bp	1.0	81.7
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGTCTGGTTGTAGCATTGG	50.0	50.0	57.9	57.8	654-680 bp	1.0	80.9
atpF-atpH_p3	CCAGTGACCCAAGAAAACGA	GCATTGGCGCTTTTATTTGC	50.0	45.0	58.0	57.6	614-640 bp	1.0	79.9
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCATTGGCGCTTTTATTTGC	50.0	45.0	57.9	57.6	641-667 bp	1.0	79.1
atpF-atpH_p5	CCAGTGACCCAAGAAAACGA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	691-717 bp	1.0	77.5