Hotspot

trnK-UUU-rps16

LSC · 4383-5055 · 673 bp

Back to Gonystylus

Pi 0.00892
MarkerSeek score 63.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
673
variable_sites
6
pi
0.00892
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC GTGGGGGTTTTTACGGAACT 55.0 50.0 57.9 58.0 807 bp 1.0 79.8
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC GTGGGGGTTTTTACGGAACT 55.0 50.0 57.9 58.0 806 bp 1.0 79.8
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GTGGGGGTTTTTACGGAACT 50.0 50.0 58.2 58.0 746 bp 1.0 79.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GTGGGGGTTTTTACGGAACT 50.0 50.0 58.2 58.0 745 bp 1.0 79.2
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GTGGGGGTTTTTACGGAACT 50.0 50.0 58.3 58.0 755 bp 1.0 78.3