Hotspot

rps16

Genome · 2388-3415 · 1028 bp

Back to Goniophlebium

Pi 0.155

MarkerSeek score 81.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 1028
variable_sites: 157
pi: 0.155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AACGCTTGCTTAACCACAGA	TTGGAGATACGATAGCCCGT	45.0	50.0	58.0	58.0	1906 bp	0.5	59.6
rps16_p2	CTCCCCCTGCTTTTCCAATT	TTGGAGATACGATAGCCCGT	50.0	50.0	58.1	58.0	1829 bp	0.5	59.5
rps16_p3	AACGCTTGCTTAACCACAGA	TTACGAAGGCACCAACCAAA	45.0	45.0	58.0	57.9	1755 bp	0.5	59.3
rps16_p4	AACGCTTGCTTAACCACAGA	TCTAGGTTACGAAGGCACCA	45.0	50.0	58.0	58.1	1761 bp	0.5	59.1
rps16_p5	CTCCCCCTGCTTTTCCAATT	TTACGAAGGCACCAACCAAA	50.0	45.0	58.1	57.9	1678 bp	0.5	59.1