Hotspot

rpl23

Genome · 80614-80880 · 267 bp

Back to Goniophlebium

Pi 0.0824

MarkerSeek score 70.30

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: Genome
length_bp: 267
variable_sites: 22
pi: 0.0824
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	CTGGCTACTCTTCCGTCAAC	CATCCATGGCTGAACGGTTA	55.0	50.0	58.0	58.0	814-817 bp	1.0	87.8
rpl23_p2	GGTACCTGGAGTATACGCCT	CATCCATGGCTGAACGGTTA	55.0	50.0	58.0	58.0	614-617 bp	1.0	87.8
rpl23_p3	CTGGCTACTCTTCCGTCAAC	TGAACGGTTAAAGCACCCAA	55.0	45.0	58.0	57.9	804-807 bp	1.0	87.6
rpl23_p4	GGTACCTGGAGTATACGCCT	GCCCGAACCTGCAGTTAATA	55.0	50.0	58.0	58.0	1021-1037 bp	1.0	87.2
rpl23_p5	CTGGCTACTCTTCCGTCAAC	GCCCGAACCTGCAGTTAATA	55.0	50.0	58.0	58.0	1221-1237 bp	1.0	87.1