Hotspot

rps12-rrn16

Genome · 138449-140360 · 1912 bp

Back to Goniophlebium

Pi 0.0403

MarkerSeek score 82.30

Primer available yes

Label rps12-rrn16

Feature Metadata

label_name: rps12-rrn16
feature_type: igs
region: Genome
length_bp: 1912
variable_sites: 76
pi: 0.0403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rrn16_p1	GAACTCTCGCGACTTTACGT	CACTGGAAACACCACGAAGA	50.0	50.0	58.0	58.1	2061-2063 bp	1.0	83.2
rps12-rrn16_p2	GAACTCTCGCGACTTTACGT	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.0	57.9	2032-2034 bp	1.0	82.9
rps12-rrn16_p3	CTCTCGCGACTTTACGTAGG	CACTGGAAACACCACGAAGA	55.0	50.0	57.9	58.1	2058-2060 bp	1.0	82.8
rps12-rrn16_p4	AGCCGTTACCTCAAACTTGG	CACTGGAAACACCACGAAGA	50.0	50.0	58.1	58.1	2090-2092 bp	1.0	82.6
rps12-rrn16_p5	GCCGTTACCTCAAACTTGGA	CACTGGAAACACCACGAAGA	50.0	50.0	58.1	58.1	2089-2091 bp	1.0	82.5