Hotspot

ycf2-ndhF

Genome · 104729-105788 · 1060 bp

Back to Goniophlebium

Pi 0.147

MarkerSeek score 82.60

Primer available yes

Label ycf2-ndhF

Feature Metadata

label_name: ycf2-ndhF
feature_type: igs
region: Genome
length_bp: 1060
variable_sites: 153
pi: 0.147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-ndhF_p1	TCGCTTTGATTCCTACGTGG	ATCAACGGAACTGGTGCATT	50.0	45.0	58.0	58.1	1439 bp	0.5	59.2
ycf2-ndhF_p2	ACATTTAATCCCGCTCGCTT	ATCAACGGAACTGGTGCATT	45.0	45.0	58.0	58.1	1453 bp	0.5	59.0
ycf2-ndhF_p3	TCCGCAGACATTTAATCCCG	ATCAACGGAACTGGTGCATT	50.0	45.0	58.1	58.1	1460 bp	0.5	58.9
ycf2-ndhF_p4	TTTAATCCCGCTCGCTTTGA	ATCAACGGAACTGGTGCATT	45.0	45.0	57.9	58.1	1450 bp	0.5	58.5
ycf2-ndhF_p5	ATTTGGGAATATCCGGCGAC	ATCAACGGAACTGGTGCATT	50.0	45.0	58.1	58.1	1848 bp	0.5	58.4