Hotspot

rrn16-rps12

Genome · 92895-94807 · 1913 bp

Back to Goniophlebium

Pi 0.0403

MarkerSeek score 82.20

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: Genome
length_bp: 1913
variable_sites: 76
pi: 0.0403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	CACTGGAAACACCACGAAGA	GAACTCTCGCGACTTTACGT	50.0	50.0	58.1	58.0	2061-2063 bp	1.0	83.2
rrn16-rps12_p2	TCCGACTTGCATGTGTTAGG	GAACTCTCGCGACTTTACGT	50.0	50.0	57.9	58.0	2032-2034 bp	1.0	82.9
rrn16-rps12_p3	CACTGGAAACACCACGAAGA	CTCTCGCGACTTTACGTAGG	50.0	55.0	58.1	57.9	2058-2060 bp	1.0	82.8
rrn16-rps12_p4	CACTGGAAACACCACGAAGA	AGCCGTTACCTCAAACTTGG	50.0	50.0	58.1	58.1	2090-2092 bp	1.0	82.6
rrn16-rps12_p5	CACTGGAAACACCACGAAGA	GCCGTTACCTCAAACTTGGA	50.0	50.0	58.1	58.1	2089-2091 bp	1.0	82.5